The Biotechnology Program's Summer Courses

Summer 2017

The Biotechnology Program offers the following short courses on various techniques used in biotechnology research. If you would like to receive training in any of the areas listed below, please register through UCD Conference and Event Services. Their link is provided below for each of the courses and by selecting it, registration will be possible for that particular class.

Register HERE


FLOW CYTOMETRY COURSE


Haring Hall
Bridget & Johnathon

Days, Dates and Times:

July 17 - 21, 2017 Monday through Friday

Lead Instructors:

Bridget McLaughlin, Technical Director, UC Davis Flow Cytometry Shared Resource

Facility:

Genome Center and Tupper Hall

This comprehensive flow cytometry course is enhanced with an exciting lineup of interactive laboratory sessions using many of our UC Davis cytometers as well as new instrumentation and software to teach you to a variety of established and new approaches in cytometry. Special guest speakers will introduce new trends in flow cytometry and explain the physical and electronic concepts in light collection and signal processing. An optional “dinner with the experts” evening is planned and lunch sessions will provide a forum for questions and interaction with the instructors.

Course objectives:

  • Learn the fundamental concepts of how flow cytometers work
  • Discuss best practices in sample preparation for flow cytometry
  • Describe standard assays and techniques
  • Introduce new cutting edge technologies and applications for single cell analysis and genotyping
  • Demystify multicolor cell staining, compensation and analysis

Laboratory sessions include:

  • UC Davis MoFlo Cell Sorter: cell sorting theory and practical considerations 
  • UC Davis LSRII: Software setup for successful multicolor acquisition and analysis
  • Multispectral imaging flow cytometry using the Amnis FlowSight Imaging Cytometer
  • Multiparameter Analysis of Cell Surface Markers, Cytokines, Transcription Factors, and Phosphoprotein Expression by Flow Cytometry: BD LSRII cytometer
  • FlowJo basic and advanced flow cytometry data analysis and panel design wizard
  • Targeted gene expression in single cells using flow sorting and the Fluidigm BioMark

Special guest speakers:

  • Holden Maecker, Ph.D., Director, Stanford University Human Immune Monitoring Core
  • Jolene Bradford, Staff Scientist, Molecular Probes/Life Technologies Cytometry Reagents
  • Marty Bigos, Director, Stanford Shared Flow Cytometry Laboratory
  • Nikesh Kotecha, Ph.D., Cytobank software solutions for flow Cytometry

Flow Cytometry Course Registration HERE

Enrollment is limited to 30 students (lecture/lab), 40 lecture only.

 

See photos of 2016 course HERE

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PROTEOMICS COURSE

Register HERE

Brett Phinney
proteomics lab


Dates:

August 7 - 11, 2017

Lead Instructor:

Brett Phinney, PhD, (UC Davis Proteomics Facility www.proteomics.ucdavis.edu , UC Davis Genome Center).

Lab Instructors: Anthony Herren, PhD and Michelle Salemi

Facility:

Genome Center and Science Lab Building (SLB)

This hands-on introductory proteome analysis workshop is designed to expose participants to fundamental technology platforms and current methodologies in the field of mass spectrometry-based proteomics.

Topics covered include:

  • Bottom-up quantitative and non-quantitative nano-electrospray proteomics tandem mass spectrometry (nESI-LC/MSMS)
  • In-gel digestion lab class
  • Nano-scale HPLC & peptide spectrum matching using open-source software (X! Tandem)
  • Targeted proteomics
  • de novo sequencing
  • Data analysis, using programs such as Scaffold and Skyline

Lectures will be presented by the proteomics core staff as well as leading proteomics experts including scientists from UC Davis, UCSF, Stanford, Bruker, Thermo Scientific and the Buck institute. This is an intensive, practical course that is meant to give the participant enough background to allow them to take the course material and build upon and apply it to their own individual projects. No proteomics experience is necessary, although laboratory experience working with proteins is usually helpful.

Specific course topics may change due to sample and mass spectrometer availability.

 

Enrollment is limited to 30 students (lecture/lab), 40 lecture only.

Proteomics Course Registration HERE

See photos of 2016 course HERE